Minding the gap: International team defines the spaces through which nerve cells communicate

NIH-funded research leads to visualization of the synaptic cleft, the space between neuronal contacts, at high resolution and finds cloud-like formations

Network of proteins that connects synapses

BOSTON (Dec. 16, 2015, noon ET)— In a report published in the journal Neuron, an international team of researchers defined the makeup of the cellular structures through which nerve cells communicate with each other. These “synaptic clefts” are the small gaps between nerve cells (neurons) that relay information in the brain. Synapses, including the synaptic clefts, are formed rapidly shortly before and after birth. Mutations in the proteins that make up the cleft increase vulnerability to developmental disorders, notably autism spectrum disorders.

The research team set out to identify the molecular and structural organization of the synaptic cleft and the role of proteins that adhere to each other to hold the cleft together. To gain insight on the molecular level, the team examined the synaptic clefts in mice by studying two of the synapse-organizing proteins. They employed an array of the most advanced imaging techniques available including tomography and imaging below the diffraction limit of light.

“By illuminating how nerve cells are connected to each other, we are closer to understanding what organizational steps go awry in synaptic disorders that impair brain development,” said senior author Thomas Biederer, Ph.D., from the Department of Neuroscience at Tufts University School of Medicine. He is also a member of the program faculties in Cell, Molecular & Developmental Biology, Cellular & Molecular Physiology, and Neuroscience at the Sackler School of Graduate Biomedical Sciences at Tufts.

This work is the first study to quantitatively define the macromolecular organization of the synaptic cleft. Taken together, the results support the novel concept that the synaptic cleft is comprised of structurally and molecularly diverse sub-compartments, and that its components can be dynamic.

“What surprised me most was the cloud-like formations of synaptic adhesion complexes at the perimeter of the cleft,” said Biederer. “These proteins form quite beautiful structures at synapses that have never been seen before. Given that there are genetic mutations in synaptic adhesion proteins that are linked to autism, these findings enable research into how these mutations change the structure and hence the function of synapses.”

Among the behavioral alterations that the researchers suspect may be related to the structure and dynamics of the synaptic cleft is the ability to learn and access memory, as well as an increased risk for drug addiction.

The first author on the study is Karen Perez de Arce, PhD, a neuroscience research associate in Biederer’s lab at Tufts. In addition to Perez de Arce and Biederer, authors on the study are Nikolas Schrod, Sarah W. R. Metzbower, Edward Allgeyer, Geoffrey K.-W. Kong, Aihui Tang, Alexander J. Krupp, Valentin Stein, Xinran Liu, Jörg Bewersdorf, Thomas A. Blanpied, and Vladan Lucic. Schrod, Kong, and Lucic are with the Department of Molecular Structural Biology at the Max Planck Institute of Biochemistry; Metzbower, Tang, Blanpied are with the Department of Physiology at the University of Maryland School of Medicine; Allgeyer, Bewersdorf, and Liu are with the Department of Cell Biology at Yale University School of Medicine, and Krupp and Stein are with Department of Physiology, Universität Bonn Medical Faculty.

Research reported in this study was supported by the Tufts Center for Neuroscience Research funded by the National Institute of Neurological Disorders and Stroke of the National Institutes of Health (NIH) under award number P30NS047243. Metzbower and Blanpied were also supported by the National Institute of Mental Health of the NIH (award numbers F31MH105105 and R01MH080046 to the University of Maryland) and Biederer by the National Institute on Drug Abuse of the NIH (award number R01 DA018928 to Tufts University).

Perez de Arce et al., Topographic Mapping of the Synaptic Cleft into Adhesive Nanodomains, Neuron (2015), http://dx.doi.org/10.1016/j.neuron.2015.11.011

About Tufts University School of Medicine and the Sackler School of Graduate Biomedical Sciences

Tufts University School of Medicine and the Sackler School of Graduate Biomedical Sciences at Tufts University are international leaders in innovative medical and population health education and advanced research. Tufts University School of Medicine emphasizes rigorous fundamentals in a dynamic learning environment to educate physicians, scientists, and public health professionals to become leaders in their fields. The School of Medicine and the Sackler School are renowned for excellence in education in general medicine, the biomedical sciences, and public health, as well as for innovative research at the cellular, molecular, and population health level. Ranked among the top in the nation, the School of Medicine is affiliated with six major teaching hospitals and more than 30 health care facilities. Tufts University School of Medicine and the Sackler School undertake research that is consistently rated among the highest in the nation for its effect on the advancement of medical and prevention science.

A note regarding the above references to the Sackler School: In December 2019, Tufts University announced that it would remove the Sackler name from all programs and facilities—including the Sackler School of Graduate Biomedical Sciences. At that time, the school was renamed the Graduate School of Biomedical Sciences.
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